| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | After the cells were cultured as spheres in the stem cell medium, they were placed in a sphere containing 2% Growth factor, starved for 24 h in the basic medium of reduced Matrigel, treated with FGF2, FGF7 (GenScript, Z03154), or FGF10 (GenScript, Z03155) (50 ng mL−1) or BMP7 (50 ng mL−1) for 24 h, before RNA or proteins were extracted for downstream experiments. | Get A Quote |
Tumor heterogeneity, the presence of multiple distinct subpopulations of cancer cells between patients or among the same tumors, poses a major challenge to current targeted therapies. The way these different subpopulations interact among themselves and the stromal niche environment, and how such interactions affect cancer stem cell behavior has remained largely unknown. Here, it is shown that an FGF-BMP7-INHBA signaling positive feedback loop integrates interactions among different cell populations, including mammary gland stem cells, luminal epithelial and stromal fibroblast niche components not only in organ regeneration but also, with certain modifications, in cancer progression. The reciprocal dependence of... More
